RNA-sequencing data

Mouse lung ECs were isolated as described previously [8,35]. Purified endothelial cells (EC, CD31⁺cells) were lysed for RNA isolation with the RNeasy mini kit (Qiagen Inc., Germantown, MD, USA) including DNase I digestion. Equal amounts of RNA from ECs isolated from three individual WT or <i>Egln1^Tie2Cre</i> mice were pooled and sequenced with NovaSeq PE150 at Novogene Corporation Inc. (Sacramento, CA, USA) The original sequencing data were trimmed using FASTX and aligned to the reference genome using TopHat2. The differential expression analysis was performed using Cuffdiff software [36].

小鼠肺内皮细胞（endothelial cells, EC）按照此前已报道的方法[8,35]完成分离。纯化得到的内皮细胞（即CD31⁺细胞）采用RNeasy迷你试剂盒（Qiagen Inc.，美国马里兰州日耳曼敦）进行裂解以提取RNA，该试剂盒包含DNase I消化步骤。将分别从3只独立的野生型（wild type, WT）或Egln1^Tie2Cre小鼠中分离的内皮细胞所提取的等量RNA混合后，于Novogene Corporation Inc.（美国加利福尼亚州萨克拉门托）采用NovaSeq PE150平台完成测序。原始测序数据经FASTX工具进行序列修剪，并通过TopHat2软件比对至参考基因组。差异表达分析采用Cuffdiff软件完成[36]。