Ecoli_Cojugation

These are donor E coli cells (ANB35) and recipient Ecoli cells (ANB24). These are the same . cells described in the Science paper (Direct visualization of horizontal gene transfer). The . cells were mixed on the grid and then plunge frozen and imaged with cryo-ET without CLEM. . Targets were imaged with cryo-ET randomly.

本数据集包含供体大肠杆菌（E. coli）菌株ANB35与受体大肠杆菌（E. coli）菌株ANB24。上述菌株与发表于《科学》（Science）期刊、题为《水平基因转移的直接可视化》的论文中所描述的细胞为同一批。这些细胞被涂布于电镜载网上混合后，经快速浸没冷冻（plunge freezing）处理，随后使用冷冻电子断层扫描（cryo-ET）进行成像，未采用关联光镜-电镜技术（CLEM）。成像靶标通过冷冻电子断层扫描（cryo-ET）进行随机成像。