DNA sequence of the gp63 fragment encoding the functional domain of leishmanolysin in natural isolates of Leishmania braziliensis from Corte de Pedra, Northeast Brazil: allele 24

DNA
sequence of the gp63 fragment encoding the functional domain of leishmanolysin
in natural isolates of Leishmania braziliensis from Corte de Pedra, Northeast
Brazil: allele 24. The stretch of gp 63 previously described as encoding the
functional domain of the molecule was amplified by PCR, cloned into pcr 2.1
vector and bidirectionally sequenced. Sequences from a panel of 41 parasite
isolates were aligned and 45 alleles of the fragment could be identified based
on indel and SNPs presented among cloned domains. Haplotypes of polymorphisms
(indel and SNPs) that could be detected in at least two clones of the gp63
domain in a single isolate, and in at least two parasite isolates in the
collection were considered alleles. Corte de Pedra is a region highly endemic
for American tegumentary leishmaniasis (ATL) due to L. braziliensis. Several
forms of ATL occur and close to 1,500 new cases are diagnosed in the
region each year.

巴西东北部科特德佩德拉（Corte de Pedra）地区巴西利什曼原虫（Leishmania braziliensis）自然分离株中，编码利什曼溶素（leishmanolysin）功能结构域的gp63片段DNA序列：等位基因24。此前被报道可编码该分子功能结构域的gp63区段，经聚合酶链式反应（PCR）扩增后，被克隆至pcr2.1载体，并完成双向测序。对41株寄生虫分离株的序列进行多序列比对后，基于克隆结构域间存在的插入缺失（insertion-deletion, indel）与单核苷酸多态性（Single Nucleotide Polymorphism, SNPs），可鉴定出该片段的45个等位基因。若某一多态性（包含插入缺失与单核苷酸多态性）的单倍型，可在单株分离株的至少两个gp63结构域克隆中被检出，且在该菌株收集库的至少两株寄生虫分离株中存在，则将其界定为等位基因。科特德佩德拉是由巴西利什曼原虫导致的美洲皮肤利什曼病（American Tegumentary Leishmaniasis, ATL）高流行区域。该地区存在多种临床类型的美洲皮肤利什曼病，每年新增确诊病例近1500例。