Table1_High Expression of FCRLB Predicts Poor Prognosis in Patients With Colorectal Cancer.XLSX

Background: Mining the prognostic biomarkers of colorectal cancer (CRC) has important clinical and scientific significance. The role of Fc receptor-like B (FCRLB) in solid tumors has never been reported or studied to our knowledge, and the prognostic role of FCRLB in CRC still awaits characterization. Methods: The potential prognostic factor FCRLB was screened out through TCGA database analysis. Then, its expression and associations with clinicopathological variables were assessed in the TCGA CRC cohort. The prognostic value of FCRLB was examined with multiple methods, such as the Kaplan-Meier method, ROC curve, time-dependent ROC analysis, and prediction model nomograms. Then, functional enrichment and annotation among the high and low FCRLB groups were achieved utilizing GO and KEGG analyses and GSEA. Fresh CRC tissue samples obtained clinically were used for the preparation of the tissue microarray and for further validation. Results: FCRLB was highly expressed in CRC tissues compared to normal tissues. Moreover, over-expression of FCRLB correlated with higher CEA levels, advanced T stage, N stage, M stage, AJCC stage, lymphatic invasion, perineural invasion, and incomplete resection (R1 and R2 resection). In addition, high expression of FCRLB was closely correlated to less favorable OS, DSS, and PFI. The analysis of CRC tissue microarray further confirmed the conclusion drawn from the TCGA data analysis. Conclusion: FCRLB is notably up-regulated in CRC tissues and may serve as a potential biomarker of CRC.背景：挖掘结直肠癌（colorectal cancer, CRC）的预后生物标志物具有重要的临床与科研价值。据我们所知，Fc受体样B（Fc receptor-like B, FCRLB）在实体瘤中的作用尚未见相关报道或研究，其在结直肠癌中的预后价值仍有待阐明。 方法：本研究通过癌症基因组图谱（The Cancer Genome Atlas, TCGA）数据库分析筛选出潜在预后因子FCRLB。随后，在TCGA结直肠癌队列中评估其表达水平及与临床病理变量的关联。采用Kaplan-Meier法、ROC曲线、时间依赖性ROC分析以及预测模型列线图等多种方法检验FCRLB的预后价值。通过基因本体（Gene Ontology, GO）、京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes, KEGG）富集分析及基因集富集分析（Gene Set Enrichment Analysis, GSEA），对FCRLB高、低表达组进行功能富集注释。收集临床获取的新鲜结直肠癌组织样本用于制备组织微阵列，以开展进一步验证。 结果：相较于正常组织，FCRLB在结直肠癌组织中呈高表达。进一步分析显示，FCRLB高表达与较高的癌胚抗原（carcinoembryonic antigen, CEA）水平、较晚的T分期、N分期、M分期、AJCC分期，以及淋巴侵袭、神经周围侵袭和不完全切除（R1、R2切除）均显著相关。此外，FCRLB高表达与更差的总生存期（overall survival, OS）、疾病特异性生存期（disease-specific survival, DSS）和无进展间期（progression-free interval, PFI）密切相关。结直肠癌组织微阵列分析进一步验证了TCGA数据分析所得结论。 结论：FCRLB在结直肠癌组织中显著上调，有望成为结直肠癌潜在的预后生物标志物。