Table8.XLSX

Circular RNAs (circRNAs) have been reported that can be used as biomarkers for colorectal cancers (CRC) and other types of tumors. However, a limited number of studies have been performed investigating the potential role of circRNAs in tumor metastasis. Here, we examined the circRNAs in two CRC cell lines (a primary tumor cell SW480 and its metastasis cell SW620), and found a large set of circRNA (2,919 ncDECs) with significantly differential expression patterns relative to normal cells (NCM460). In addition, we uncovered a set of 623 pmDECs that differ between the primary CRC cells and its metastasis cells. Both differentially expressed circRNA (DEC) sets contain many previously unknown putative CRC-related circRNAs, thereby providing many new circRNAs as candidate biomarkers for CRC development and metastasis. These studies are the first large-scale identification of metastasis-related circRNAs for CRC and provide valuable candidate biomarkers for diagnostic and a starting point for additional investigations of CRC metastasis.

已有研究表明，环状RNA（Circular RNAs, 缩写circRNAs）可作为结直肠癌（colorectal cancers, 简称CRC）及其他肿瘤的生物标志物。然而，目前针对环状RNA在肿瘤转移中潜在作用的研究仍较为有限。本研究对两株结直肠癌细胞系（原代肿瘤细胞SW480及其转移细胞SW620）中的环状RNA进行了分析，发现相较于正常细胞NCM460，存在2919个ncDECs，其表达模式存在显著差异。此外，本研究还鉴定出623个在原代结直肠癌细胞与转移细胞之间存在表达差异的pmDECs。上述两组差异表达环状RNA（differentially expressed circRNA, 简称DEC）均包含大量此前未被报道的疑似结直肠癌相关环状RNA，从而为结直肠癌发生与转移的候选生物标志物提供了诸多新的环状RNA靶点。本研究首次对结直肠癌转移相关环状RNA开展了大规模鉴定工作，可为结直肠癌的临床诊断提供极具价值的候选生物标志物，同时也为结直肠癌转移的后续研究奠定了重要基础。