Nanoparticle protein corona LC-MS/MS

Engineered nanoparticles for biomedical applications require increased effectiveness in targeting specific cells while preserving non-target cells’ safety. Native nanoparticles entering a protein-rich liquid media quickly form a macromolecular structure called protein corona. This protein structure defines the physical interaction between nanoparticles and target cells. We describe SUSTU (surface proteomics for nanoparticle safety, targeting and uptake) a proteomics-based method to analyze the surface of a nanoparticle protein corona. This method provides qualitative and quantitative analysis from the protein corona surface. Those exposed proteins compose the first line of interaction between this macromolecular structure and the cell surface of a target cell. With SUSTU, the spatial and temporal dynamics of the protein corona surface can be studied. Data from SUSTU would ascertain the nanoparticle functionalized groups exposed at destiny; circumventing preliminary in vitro experiments. Therefore this method evaluates nanoparticle targeting and uptake capability and could be integrated into a rapid prototyping strategy which is a major challenge in nanomaterial science.

用于生物医学应用的工程化纳米颗粒，需在提升对特定细胞靶向效能的同时，保障非靶向细胞的使用安全性。未修饰的纳米颗粒进入富蛋白液体培养基后，会快速形成一种名为蛋白冠（protein corona）的大分子结构。该蛋白结构决定了纳米颗粒与靶细胞之间的物理相互作用。本研究介绍了SUSTU（纳米颗粒安全性、靶向性与摄取的表面蛋白质组学，surface proteomics for nanoparticle safety, targeting and uptake）：一种基于蛋白质组学的纳米颗粒蛋白冠表面分析方法。该方法可对蛋白冠表面进行定性与定量分析。这些暴露于表面的蛋白，构成了该大分子结构与靶细胞表面相互作用的第一道界面。借助SUSTU方法，可研究蛋白冠表面的时空动态变化特征。SUSTU产生的数据可确定纳米颗粒功能化基团在靶位点的暴露情况，从而规避前期体外实验环节。因此，该方法可评估纳米颗粒的靶向性与摄取能力，且可集成于快速原型开发策略中——这也是纳米材料科学领域的一大核心挑战。