Towards 3D structure determination and dynamics of biological and non-biological samples using TR-SSX methods

This BAG proposal aims to conduct room temperature time-resolved serial crystallography experiments at the new ID29 beamline at ESRF. We intend to collect full data sets on microcrystals of two medically relevant proteins, the penicillin binding protein 2a (PBP2a) of methicillin resistant staphyloccocus aureus (MRSA) and the human the NAP(P)H:quinone oxidoreductase 1 (NQO1) protein, with their respective ligands. As well, we aim at conducting pump-probe time-resolved crystallography experiments on microcrystals of metal organic frameworks (MOFs). The good quality of all crystalline samples has been proved either at ESRF beamlines ID30-A1 and ID30-A3, or at other synchrotron beamlines. At ID29, they offer a variety of sample delivery devices including high viscosity injectors, tape drives, fixed target supports, and microfluidic devices, that will be employed in our experiments depending on sample needs.

本BAG提案旨在欧洲同步辐射装置 (ESRF) 新建的ID29光束线开展室温时间分辨串行晶体学实验。我们计划采集两种医学相关蛋白的微晶完整数据集：分别为耐甲氧西林金黄色葡萄球菌 (MRSA) 的青霉素结合蛋白2a (PBP2a)，以及人类NAP(P)H：醌氧化还原酶1 (NQO1)，并结合其各自配体完成数据采集。此外，我们还计划针对金属有机框架 (MOFs) 的微晶开展泵浦-探测型时间分辨晶体学实验。所有晶体样品的优异品质已在ESRF的ID30-A1、ID30-A3光束线及其他同步辐射光束线上得到验证。ID29光束线配备多种样品递送装置，涵盖高粘度注射器、胶带传送系统、固定靶支架及微流控设备，我们将根据样品的实际需求在实验中选用适配的装置。