Impact of mononuclear cell subset depletion on IFNγ producing CD8⁺ T cells (brain infiltrates).

Six days after infection, cellular infiltrates from the brains of individual mice were prepared and analysed by flow cytometry for the frequency of infiltrating CD8+ cells which produce IFNγ or not upon phorbol myristate acetate (PMA) /Ionomycin restimulation. a n = 4–5 per group b Significant differences between PbTg infected group and naïve mice, (p<0.05, Mann-Whitney U test) c Significant differences between mAb-depleted versus non-depleted PbTg infected mice, (p<0.05, Mann-Whitney U test) Impact of mononuclear cell subset depletion on IFNγ producing CD8+ T cells (brain infiltrates).感染后第6天，分离制备各组小鼠脑组织的细胞浸润组分，采用流式细胞术（flow cytometry）分析经佛波醇肉豆蔻酸乙酸酯（phorbol myristate acetate, PMA）/离子霉素（Ionomycin）复刺激后，浸润性CD8+细胞中产生干扰素γ（IFNγ）与不产生该细胞因子的细胞占比。 a 每组样本量为4~5只。 b PbTg感染组与未致敏小鼠组间存在显著统计学差异（p<0.05，曼-惠特尼U检验）。 c 单克隆抗体（monoclonal antibody, mAb）耗竭组与未耗竭的PbTg感染小鼠组间存在显著统计学差异（p<0.05，曼-惠特尼U检验）。 单核细胞亚群耗竭对脑内浸润性IFNγ产生型CD8+ T细胞的影响。