BF_LN_OVS1_NS

To assess changes in immune gene expression following HIV-env vaccination through 3 delivery systems, rhesus macaques were vaccinated with SOSIP trimer with adjuvants using either oral, intraepithelial delivery through a syringe (IEP), a needle free pressurized oral delivery (NF), or an intramuscular injection in the right deltoid (IM). Submandibular lymph nodes were biopsied and stored in RPMI supplemented with 10% fetal bovine serum. RNA was extracted using Macherey Nagel nucleospin columns. RNA from both timepoints was then used for gene expression analysis using the Nanostring nCounter platform. Data from 9 adult macaques was obtained from samples representing time points 2 weeks prior to, and 1 week following, vaccination

为评估三种递送系统接种HIV-env疫苗后免疫基因表达的变化，研究人员对恒河猴接种含佐剂（adjuvants）的SOSIP三聚体（SOSIP trimer）疫苗，接种方式涵盖口服、无针加压口服递送（NF）、注射器辅助上皮内递送（IEP）以及右侧三角肌肌内注射（IM）。研究人员采集下颌下淋巴结样本进行活检，并将样本保存于添加10%胎牛血清的RPMI培养基中。随后使用Macherey Nagel Nucleospin柱式试剂盒提取RNA，再通过Nanostring nCounter平台对两个时间点的RNA开展基因表达分析。本研究最终获取了9只成年恒河猴在疫苗接种前2周及接种后1周两个时间点的样本数据。