Inhibition of pyrimidine biosynthesis targets protein translation in AML [ATAC-seq_MN-2]

Investigation of the chromatin accessibility in MN cells. Mice bearing MN tumors were treated with AG636 for 2 days. Leukemic stem cells (cKit high; CD11b low) from bone marrow and spleen were isolated. RNA sequencing and ATAC-seq were performed. Overall design: Genome binding/occupancy profiling of Vehicle- or AG636-treated MN AML cells by high-throughput sequencing.

MN细胞染色质可及性研究。携带MN肿瘤的小鼠经AG636处理2天。从骨髓与脾脏中分离得到白血病干细胞（cKit高表达；CD11b低表达）。开展RNA测序与ATAC-seq实验。实验设计：通过高通量测序对赋形剂（Vehicle）或AG636处理的MN急性髓系白血病（AML）细胞进行基因组结合/占据谱分析。