Comparison of RNA-Seq and Microarray in Transcriptome Profiling During T Cell Activation

Samples in this study probe the gene expression kinetics in human CCR6+ Th17 memory T cells activated under Th17 condition. Human CCR6+ Th17 memory T cells were purified from PBMC and gene expression was studied over a time course of 3 days after activation under Th17 condition. RNA from these samples was also profiled using RNA-Seq to compare different transcriptome profiling technologies. PBMCs were purified from whole blood from a healthy donor. CD4+ cells were isolated by negative selection, followed by CCR6+ purification by positive selection. Two replicates of 6 time points were taken following stimulation in Th17 polarizing conditions. This submission represents microarray component of study.

本研究中的样本旨在探究在Th17极化条件下激活的人CCR6+ Th17记忆T细胞的基因表达动力学。研究使用的人CCR6+ Th17记忆T细胞从外周血单个核细胞（PBMC, Peripheral Blood Mononuclear Cell）中纯化得到，随后在Th17极化条件下激活，并对其基因表达进行了为期3天的时序分析。同时，还通过RNA测序（RNA-Seq）对上述样本的RNA进行了转录组分析，以对比不同转录组分析技术的表现。本研究的外周血单个核细胞取自一名健康志愿者的全血并完成纯化：首先通过阴性分选法分离CD4+ T细胞，随后通过阳性分选法纯化得到CCR6+细胞。在Th17极化条件下刺激细胞后，共设置6个时间点，每个时间点设置2个生物学重复。本次提交的数据集为本研究的基因芯片（microarray）分析部分。