Trichostatin A does not correct specific errors of somatic cell nuclear transfer on the transcriptomic level; highlighing the non-random nature of oocyte-mediated reprogramming errors.. Bos taurus

Bovine cloned and IVF blastocysts at day 7 were collected and used for comparative transcriptomics. Overall design: A potent histone deacetylase inhibitor, Trichostatin A (TSA), was used to understand the effect of assisted epigenetic modification on the transcriptional profile of SCNT blastocysts and to identify specific genes or categories of genes affected in the developing embryos.

收集第7天的牛克隆胚胎与体外受精（In Vitro Fertilization, IVF）囊胚，用于比较转录组学研究。实验设计：采用强效组蛋白去乙酰化酶抑制剂曲古抑菌素A（Trichostatin A, TSA），以探究辅助性表观遗传修饰对体细胞核移植（Somatic Cell Nuclear Transfer, SCNT）囊胚转录谱的影响，并鉴定发育胚胎中受影响的特异性基因或基因类别。