SH1044282.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE平台为界定、识别、交流以及处理基于DNA的物种假说（DNA-based Species Hypotheses，以下简称SH）提供了统一的操作框架。国际核酸序列数据库中的所有真菌内转录间隔区（Internal Transcribed Spacer，ITS）序列，均通过应用一组动态距离阈值（0.5%~3.0%）聚类至近似物种级别的聚类簇。所有物种假说均会被赋予一个以数字对象标识符（Digital Object Identifier，DOI）为形式的唯一且稳定的名称，其分类学与生态学注释信息将通过分布式的网络第三方注释工作完成核验。所有SH均会结合簇内全部序列的鉴定结果，尽可能关联至对应的分类单元名称及其分类层级（如门、纲、目等）。研究人员会选取一条经自动或人工标注的序列，作为每个SH的代表序列。上述代表序列已通过链接（https://unite.ut.ee/repository.php）向全球科研社群公开，可用于本地序列相似性搜索、下一代测序（Next-Generation Sequencing）分析流程等研究场景。随着公共真菌ITS序列数据量的持续增长，该系统与数据集也将自动完成更新。