Transcriptomes of CD34+ hematopoietic stem/progenitor cells (HSPCs) from myelodysplastic syndromes (MDS) patients cultured in normoxia or hypoxia.

Three MDS patients (SF3B1mut -H662/H662/Q699) and three age-matched healthy donors were challenged in colony forming assay under normoxic (20% O2) and hypoxic (3% O2) conditions. RNA-sequencing was performed on the HSPC-derived colonies o examine transcriptional changes under normoxic and hypoxic conditions. Overall design: RNA-sequencing was performed on CD34+ hematopoietic stem/progenitor cells (HSPCs) from myelodysplastic syndromes (MDS) patients or Healthy donors in normoxia and hypoxia, n = 3 per group.

本研究纳入3例携带SF3B1突变（突变位点为H662、H662、Q699）的骨髓增生异常综合征（Myelodysplastic Syndromes, MDS）患者，以及3例年龄匹配的健康供者，将两组样本分别置于常氧（20% O₂）与低氧（3% O₂）条件下开展集落形成试验。随后对造血干/祖细胞（Hematopoietic Stem/Progenitor Cells, HSPC）来源的细胞集落进行RNA测序，以检测常氧与低氧条件下的转录组变化。实验整体设计：分别采集MDS患者与健康供者的CD34阳性造血干/祖细胞，在常氧及低氧环境下进行RNA测序，每组设置3个生物学重复。