scRNAseq of Notch signaling responsive and unresponsive bone marrow lymphoid-primed multipotent progenitors (BM LMPPs)

We report the gene expression profile of single cell BM LMPPs that are either responsive to Notch signals, or unresponsive to Notch signals due to absence of expression of RBPJ, a key mediator of the Notch signaling pathway. BM LMPPs were FACS sorted from control mice (always maintained expression of RBPJ), RBPJ uninduced mice (lacked expression of RBPJ), and RBPJ induced mice (initially lacked expression of RBPJ but made to induce expression of RBPJ through doxycycline treatment).

本研究报道了两类单细胞骨髓淋巴髓系多能祖细胞（BM LMPPs）的基因表达谱：一类可响应Notch信号，另一类因缺失Notch信号通路关键介导因子RBPJ的表达而无法响应Notch信号。实验中，研究人员通过荧光激活细胞分选术（FACS）分别从三组小鼠中分离得到上述细胞：对照组小鼠（始终维持RBPJ的表达）、RBPJ未诱导组小鼠（无RBPJ表达），以及RBPJ诱导组小鼠（初始状态下无RBPJ表达，经多西环素（doxycycline）处理后可诱导RBPJ表达）。