mRNAs and miRNAs expression data from AOM/DSS, AOM, DSS and control mouse colon epithelial tissue at day100 when tumor formed in AOM/DSS bearing mice --- mRNA expression data

To find out which mRNAs are significantly differential expression and potentially involved in the process of inflammation promoting carcinogenesis of colorectal cancer (CRC). We established a colitis-associated CRC (AOM/DSS, Azoxymethane/Dextran sulfate sodium salt) model, colitis (DSS) model and high dose carcinogen (AOM, about 5 times AOM amount given than AOM/DSS model) model. At day 100 when tumor formed in AOM/DSS bearing mice (colitis-associated CRC mice) but no tumor was found in AOM (high dose carcinogen) and DSS model, we employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to involve in the progression of CRC promoted by inflammation. 5-7 weeks female BALB/c mice, (1) AOM/DSS group: AOM 12.5mg/kg i.p. at day 1, DSS drinking 5d/21dx3circles from day 5; (2) AOM group: AOM 10mg/kg i.p. 1/weekx6 from day 1; (3) DSS group: DSS drinking 5d/21dx3circles from day 5. The distal colon epithelial tissues were collected at day100 when tumor formed in AOM/DSS bearing mice. The whole genome microarray expression profiling experiments were performed together.

本研究旨在筛选在结直肠癌（colorectal cancer, CRC）炎症促癌变进程中发挥潜在作用且存在显著差异表达的信使RNA（messenger RNA, mRNA）。我们构建了三类小鼠模型：结肠炎相关结直肠癌模型（即偶氮甲烷/葡聚糖硫酸钠（Azoxymethane/Dextran sulfate sodium salt, AOM/DSS）模型）、单纯结肠炎模型（DSS模型）以及高剂量致癌物模型（AOM模型，其给药AOM的剂量约为AOM/DSS模型中AOM剂量的5倍）。当AOM/DSS模型组（结肠炎相关结直肠癌模型小鼠）于造模后第100天成瘤时，高剂量AOM模型组与单纯DSS模型组均未检出肿瘤，此时我们以全基因组微阵列表达谱作为筛选平台，鉴定具有参与炎症介导的结直肠癌进展潜力的功能基因。实验动物为5~7周龄雌性BALB/c小鼠，具体分组及造模方案如下：（1）AOM/DSS组：于造模第1天腹腔注射12.5mg/kg AOM，自第5天起给予DSS饮水，按5天给药/21天休息的周期循环3次；（2）AOM组：自造模第1天起，每周腹腔注射10mg/kg AOM，连续给药6次；（3）DSS组：自造模第5天起给予DSS饮水，按5天给药/21天休息的周期循环3次。于造模后第100天，即AOM/DSS模型组小鼠成瘤时，采集各组小鼠的远端结肠上皮组织，统一开展全基因组微阵列表达谱实验。