Selective modulation of gene expression in activated normal human peripheral blood mononuclear cells by store-operated calcium entry blocker BTP2. Selective modulation of gene expression in activated normal human peripheral blood mononuclear cells by store-operated calcium entry blocker BTP2

Calcium is a critical signaling molecule in many cell types including immune cells. The calcium-release activated calcium channels (CRAC) responsible for store-operated calcium entry (SOCE) in immune cells are gated by STIM family members functioning as sensors of Ca2+ store content in the endoplasmic reticulum. We investigated the effect of SOCE blocker BTP2 on human peripheral blood mononuclear cells (PBMC) stimulated with the mitogen phytohemagglutinin (PHA). Overall design: To investigate the effect of SOCE blocker BTP2 on human peripheral blood mononuclear cells (PBMC) stimulated with the mitogen phytohemagglutinin (PHA), we performed RNA sequencing (RNA-seq) to query gene expression at the whole transcriptome level and identified genes differentially expressed between PBMC activated with PHA and PBMC activated with PHA in the presence of BTP2.

钙是包括免疫细胞在内的诸多细胞类型中的关键信号分子。介导免疫细胞钙池操纵性钙内流（store-operated calcium entry, SOCE）的钙释放激活钙通道（calcium-release activated calcium channels, CRAC），由作为内质网内Ca²+储库含量感受器的STIM家族蛋白完成门控调控。本研究考察了SOCE抑制剂BTP2对经有丝分裂原植物血凝素（phytohemagglutinin, PHA）刺激的人外周血单个核细胞（human peripheral blood mononuclear cells, PBMC）的作用。实验整体设计：为明确SOCE抑制剂BTP2对经PHA刺激的人PBMC的影响，我们采用RNA测序（RNA sequencing, RNA-seq）在全转录组层面检测基因表达，并鉴定了单纯经PHA激活的PBMC与经PHA激活且联合BTP2处理的PBMC之间的差异表达基因。