High Throughput Sequencing of a Mouse Model for Benign Prostatic Hyperplasia (BPH). High Throughput Sequencing of a Mouse Model for Benign Prostatic Hyperplasia (BPH)

The goal of this study is to identify genes of interest through high throughput sequencing of a mouse model for BPH and compare with control, WT mice. Additionally, to use the generated RNA expression profile to compare with a previously established human microarray for BPH. Through these comparisons we hope to identify novel genes contributing to the disease in humans. Specific results of these analyses are in progress and publication content is yet to be determined. Overall design: An RNA expression profile of the anterior prostate (AP), dorsal lateral prostate (DLP), and urethra, from control and T+E2 treated mice and from the urogogenital sinus (UGS) of E16.5 mice cultured in testosterone and without hormone. Four samples were used for each tissue in each group (control and T+E2 treated or cultured in testosterone ), making 8 samples per tissue type. All samples labelled "control" recieved no treatment.

本研究旨在通过对良性前列腺增生（BPH）小鼠模型开展高通量测序以筛选目标基因，并与野生型（WT）对照小鼠进行比对。此外，本研究将利用所获得的RNA表达谱，与已构建的良性前列腺增生人类微阵列（microarray）数据集进行比对。通过上述两类比对，我们期望筛选出与人类该疾病相关的新型致病基因。目前相关分析的具体结果仍在推进中，待发表的研究内容尚未确定。实验设计概述：采集经T+E2处理的对照组小鼠的前前列腺（AP）、背外侧前列腺（DLP）及尿道组织，以及经睾酮培养和无激素培养的E16.5小鼠尿生殖窦（UGS）的样本；每组（对照组、T+E2处理组或睾酮培养组）的每种组织均设置4个生物学重复，因此每种组织类型共计8个样本。所有标注为"control"的样本均未接受任何处理。