ARID3a is Required for Erythrocyte Lineage Differentiation of Hemin Stimulated Human K562 Cells. ARID3a is Required for Erythrocyte Lineage Differentiation of Hemin Stimulated Human K562 Cells

Inhibition of ARID3a by shRNA in hemin-induced K562 cells results in significant decrease of erythroid specific lineage factors and alpha-globin genes. Overall design: Differential expression analysis of K562 cells treated with hemin and hemin + ARID3a shRNA. For ATAC-seq samples, AL and BH represent the KO or "--" and BH and P represent wild type or "++" clones.

在血红素（hemin）诱导的K562细胞中，通过短发夹RNA（shRNA）抑制ARID3a的表达，会显著降低红系特异性谱系因子与α-珠蛋白基因的表达水平。整体实验设计：本研究对经血红素处理的K562细胞，以及经血红素联合ARID3a shRNA处理的K562细胞开展差异表达分析。针对ATAC-seq（转座酶可及性高通量测序）样本，AL与BH代表敲除（KO，即"--"）克隆，BH与P代表野生型（即"++"）克隆。