Genome-scale DNA methylation maps of mouse chromaffin cells with or without Sdhb knock-out.. Mus musculus

We report the analysis of DNA methylation in mouse chromaffin cell lines using reduced representation bisulfite sequencing (RRBS). We compared DNA methylation profiles of cell lines with or without a knock-out of Sdhb gene, showing that Sdhb disruption results in a hypermethylator phenotype. Overall design: Reduced representation bisulfite sequencing of 4 mouse chromaffin cell samples (2 Sdhb wild-type and 2 Sdhb knock-out).

本研究报道了采用简化代表性亚硫酸氢盐测序（reduced representation bisulfite sequencing, RRBS）分析小鼠嗜铬细胞系DNA甲基化的相关工作。本研究对比了存在Sdhb基因敲除与未敲除Sdhb基因的细胞系的DNA甲基化谱，结果显示Sdhb基因缺失会诱导产生高甲基化表型。实验设计：对4份小鼠嗜铬细胞样本开展简化代表性亚硫酸氢盐测序，其中2份为Sdhb野生型样本，2份为Sdhb基因敲除型样本。