Transcriptome Profiling of Human Monocyte-Derived Macrophages Upon CCL2 Neutralization Reveals an Association Between Activation of Innate Immune Pathways and Restriction of HIV-1. Transcriptome Profiling of Human Monocyte-Derived Macrophages Upon CCL2 Neutralization Reveals an Association Between Activation of Innate Immune Pathways and Restriction of HIV-1

We used RNA-sequencing to deeply characterize the cellular factors and pathways modulated by CCL2 blocking in MDMs and involved in HIV-1 replication restriction. Gene differential expression analysis was performed and then Functional annotation was done to up-regulated genes Reads that failed mapping to human genome was mapped to HIV-1 reference genome. Note that some samples in this experiment have no raw files as the data has been lost.

本研究采用RNA测序（RNA-sequencing）技术，对单核细胞衍生巨噬细胞（monocyte-derived macrophages, MDMs）中经CC趋化因子配体2（CCL2）阻断调控、且参与人类免疫缺陷病毒1型（HIV-1）复制限制过程的细胞因子与信号通路进行深度表征。随后开展基因差异表达分析，并对上调基因进行功能注释。将未能比对至人类基因组的测序读段（Reads）比对至HIV-1参考基因组。请注意，本实验中部分样本因数据丢失，暂未提供原始文件。