LPAR signaling activated in neural crest stem cells promotes melanoma cell growth, invasion and therapy resistance [HumanHT-12 V4.0]. Homo sapiens

LPAR1 (lysophosphatidic acid receptor 1) is identified in targeted siRNA screens which is required for the survival and maintenance of nueral crest stem cells (NCSC) as well as the growth and invasion of melanoma cells. To gain mechanistic insights into how LPAR signaling modulates melanoma cell growth, We conducted an Illumina genome-wide gene expression microarray experiment to profile melanoma cells treated with the autotaxin inhibitor, HA130. Melanoma cells treated with vehicle control,DMSO is included as a control. Overall design: The analysis was done in melanoma cell line, WM9, treated with DMSO or HA130.

溶血磷脂酸受体1（lysophosphatidic acid receptor 1，LPAR1）通过靶向小干扰RNA（small interfering RNA，siRNA）筛选被鉴定出，其对于神经嵴干细胞（neural crest stem cells，NCSC）的存活与维持，以及黑色素瘤细胞的增殖与侵袭均不可或缺。为深入解析LPAR信号通路调控黑色素瘤细胞增殖的分子机制，我们开展了Illumina全基因组基因表达芯片实验，对经自分泌运动因子抑制剂HA130处理的黑色素瘤细胞进行基因表达谱分析。本实验设置了溶剂对照：以二甲基亚砜（dimethyl sulfoxide，DMSO）处理的细胞作为对照。整体实验设计：本分析以WM9黑色素瘤细胞系为研究对象，分别采用DMSO或HA130进行处理。