Chromium(III) Experiment Data from Enriched Nitrifying Sludge

Raw data for Cr(III) 5 mg/L experiment.

ONTOLOGY SOURCE REFERENCE

Term Source Name
ENVO
ENVO

Term Source File
http://purl.obolibrary.org/obo/ENVO_00002046
http://purl.obolibrary.org/obo/ENVO_00002046

Term Source Description
The Environment Onthology
The Environment Onthology

STUDY

Study Identifier
Cr(III) 5 mg/L
Cr(III) 5 mg/L

Study Title
Chromium(III) impact experiments
Chromium(III) impact experiments

Study Description
Determination of nitrification kinetics, microbial abundance, and functional characteristics after Cr(III) dosage.
Determination of nitrification kinetics, microbial abundance, and functioanl characteristics after Cr(III) dosage.

Study Submission Date
2.03.2025
2.03.2025

STUDY DESIGN DESCRIPTORS

Study Design Type
aerobic bioreactor
aerobic bioreactor

Study Design Type Term Accession Number
http://purl.obolibrary.org/obo/ENVO_00002126
http://purl.obolibrary.org/obo/ENVO_00002126

Study Design Type Term Source REF
ENVO
ENVO

STUDY FACTORS

Study Factor Name
oxygen
substrate

Study Factor Type
chemical compound
chemical compound

Study Factor Type Term Accession Number
http://purl.obolibrary.org/obo/OMIT_0011105
http://purl.obolibrary.org/obo/OMIT_0002057

Study Factor Type Term Source REF
OMIT
OMIT

STUDY ASSAYS

Study Assay File Name
a_Cr_5_OUR.txt
a_Cr_5_Nitrogencompounds.txt

Study Assay Measurement Type
aerobic respiration, using ammonia as electron donor, aerobic respiration, using nitrite as electron donoraerobic respiration
concentration of nitrate and nitrite in liquid water

Study Assay Measurement Type Term Accession Number
http://purl.obolibrary.org/obo/GO_0019409, http://purl.obolibrary.org/obo/GO_0019332
http://purl.obolibrary.org/obo/ENVO_03000123

Study Assay Measurement Type Term Source REF
ENVO
ENVO

Study Assay Technology Type
respirometry
NH4-N, NO2-N, NO3-N measurement

STUDY PROTOCOLS

Study Protocol Name
respirometry
ammonia nitrogen determination, nitrite nitrogen determination, nitrate nitrogen determination

Study Protocol Description
Experimental setup was designed to have F/M ratio of 0.024 mg NH₄⁺-N/L/mg VSS by feeding activated sludge taken from lab scale reactor with 30 mg NH₄⁺-N/L ammonia nitrogen along with Cr(III), macro and micro-nutrients. Sartorius Biostat-B/Univessel reactor equipped with water jacket and MFCS software was used for dissolved oxygen measurements and OUR was calculated.
Experimental setup was designed to have F/M ratio of 0.024 mg NH₄⁺-N/L/mg VSS by feeding activated sludge taken from lab scale reactor with 30 mg NH₄⁺-N/L ammonia nitrogen along with Cr(III), macro and micro-nutrients. Nitrogen measurements were conducted in Sartorius Biostat-B/Univessel reactor equipped with water jacket and MFCS software. Samples were filtered through 0,22Um filters.

Study Protocol Parameters Name
oxygen uptake rate

NH4-N, NO2-N, NO3-N

Raw data for Cr(III) 10 mg/L experiment.

ONTOLOGY SOURCE REFERENCE

Term Source Name
ENVO
ENVO

Term Source File
http://purl.obolibrary.org/obo/ENVO_00002046
http://purl.obolibrary.org/obo/ENVO_00002046

Term Source Description
The Environment Onthology
The Environment Onthology

STUDY

Study Identifier
Cr(III) 10 mg/L
Cr(III) 10 mg/L

Study Title
Chromium(III) impact experiments
Chromium(III) impact experiments

Study Description
Determination of nitrification kinetics, microbial abundance, and functional characteristics after Cr(III) dosage.
Determination of nitrification kinetics, microbial abundance, and functional characteristics after Cr(III) dosage.

Study Submission Date
2.03.2025
2.03.2025

STUDY DESIGN DESCRIPTORS

Study Design Type
aerobic bioreactor
aerobic bioreactor

Study Design Type Term Accession Number
http://purl.obolibrary.org/obo/ENVO_00002126
http://purl.obolibrary.org/obo/ENVO_00002126

Study Design Type Term Source REF
ENVO
ENVO

STUDY FACTORS

Study Factor Name
oxygen
substrate

Study Factor Type
chemical compound
chemical compound

Study Factor Type Term Accession Number
http://purl.obolibrary.org/obo/OMIT_0011105
http://purl.obolibrary.org/obo/OMIT_0002057

Study Factor Type Term Source REF
OMIT
OMIT

STUDY ASSAYS

Study Assay File Name
a_Cr_10_OUR.txt
a_Cr_10_Nitrogencompounds.txt

Study Assay Measurement Type
aerobic respiration, using ammonia as electron donor, aerobic respiration, using nitrite as electron donoraerobic respiration
concentration of nitrate and nitrite in liquid water

Study Assay Measurement Type Term Accession Number
http://purl.obolibrary.org/obo/GO_0019409, http://purl.obolibrary.org/obo/GO_0019332
http://purl.obolibrary.org/obo/ENVO_03000123

Study Assay Measurement Type Term Source REF
ENVO
ENVO

Study Assay Technology Type
respirometry
NH4-N, NO2-N, NO3-N measurement

STUDY PROTOCOLS

Study Protocol Name
respirometry
ammonia nitrogen determination, nitrite nitrogen determination, nitrate nitrogen determination

Study Protocol Description
Experimental setup was designed to have F/M ratio of 0.024 mg NH₄⁺-N/L/mg VSS by feeding activated sludge taken from lab scale reactor with 30 mg NH₄⁺-N/L ammonia nitrogen along with Cr(III), macro and micro-nutrients. Sartorius Biostat-B/Univessel reactor equipped with water jacket and MFCS software was used for dissolved oxygen measurements and OUR was calculated.
Experimental setup was designed to have F/M ratio of 0.024 mg NH₄⁺-N/L/mg VSS by feeding activated sludge taken from lab scale reactor with 30 mg NH₄⁺-N/L ammonia nitrogen along with Cr(III), macro and micro-nutrients. Nitrogen measurements were conducted in Sartorius Biostat-B/Univessel reactor equipped with water jacket and MFCS software. Samples were filtered through 0,22Um filters.

Study Protocol Parameters Name
oxygen uptake rate
NH4-N, NO2-N, NO3-N

Raw data for Cr(III) 15 mg/L experiment.

ONTOLOGY SOURCE REFERENCE

Term Source Name
ENVO
ENVO

Term Source File
http://purl.obolibrary.org/obo/ENVO_00002046
http://purl.obolibrary.org/obo/ENVO_00002046

Term Source Description
The Environment Onthology
The Environment Onthology

STUDY

Study Identifier
Cr(III) 20 mg/L
Cr(III) 20 mg/L

Study Title
Chromium(III) impact experiments
Chromium(III) impact experiments

Study Description
Determination of nitrification kinetics, microbial abundance, and functional characteristics after Cr(III) dosage.
Determination of nitrification kinetics, microbial abundance, and functional characteristics after Cr(III) dosage.

Study Submission Date
2.03.2025
2.03.2025

STUDY DESIGN DESCRIPTORS

Study Design Type
aerobic bioreactor
aerobic bioreactor

Study Design Type Term Accession Number
http://purl.obolibrary.org/obo/ENVO_00002126
http://purl.obolibrary.org/obo/ENVO_00002126

Study Design Type Term Source REF
ENVO
ENVO

STUDY FACTORS

Study Factor Name
oxygen
substrate

Study Factor Type
chemical compound
chemical compound

Study Factor Type Term Accession Number
http://purl.obolibrary.org/obo/OMIT_0011105
http://purl.obolibrary.org/obo/OMIT_0002057

Study Factor Type Term Source REF
OMIT
OMIT

STUDY ASSAYS

Study Assay File Name
a_Cr20_OUR.txt
a_Cr_20_Nitrogencompounds.txt

Study Assay Measurement Type
aerobic respiration, using ammonia as electron donor, aerobic respiration, using nitrite as electron donoraerobic respiration
concentration of nitrate and nitrite in liquid water

Study Assay Measurement Type Term Accession Number
http://purl.obolibrary.org/obo/GO_0019409, http://purl.obolibrary.org/obo/GO_0019332
http://purl.obolibrary.org/obo/ENVO_03000123

Study Assay Measurement Type Term Source REF
ENVO
ENVO

Study Assay Technology Type
respirometry
NH4-N, NO2-N, NO3-N measurement

STUDY PROTOCOLS

Study Protocol Name
respirometry
ammonia nitrogen determination, nitrite nitrogen determination, nitrate nitrogen determination

Study Protocol Description
Experimental setup was designed to have F/M ratio of 0.024 mg NH₄⁺-N/L/mg VSS by feeding activated sludge taken from lab scale reactor with 30 mg NH₄⁺-N/L ammonia nitrogen along with Cr(III), macro and micro-nutrients. Sartorius Biostat-B/Univessel reactor equipped with water jacket and MFCS software was used for dissolved oxygen measurements and OUR was calculated.
Experimental setup was designed to have F/M ratio of 0.024 mg NH₄⁺-N/L/mg VSS by feeding activated sludge taken from lab scale reactor with 30 mg NH₄⁺-N/L ammonia nitrogen along with Cr(III), macro and micro-nutrients. Nitrogen measurements were conducted in Sartorius Biostat-B/Univessel reactor equipped with water jacket and MFCS software. Samples were filtered through 0,22Um filters.

Study Protocol Parameters Name
oxygen uptake rate
NH4-N, NO2-N, NO3-N

三价铬（Cr(III)）浓度为5 mg/L实验的原始数据。

## 本体来源参考
术语来源名称：ENVO
术语来源文件：http://purl.obolibrary.org/obo/ENVO_00002046
术语来源描述：环境本体（Environment Ontology，原文笔误为Onthology）

## 实验
实验标识：Cr(III) 5 mg/L
实验标题：三价铬影响实验
实验描述：测定投加三价铬后的硝化动力学、微生物丰度及功能特性（原文存在笔误“functioanl”，已修正为“functional”）
提交日期：2025年3月2日（原文为欧洲格式2.03.2025）

## 实验设计描述
实验设计类型：好氧生物反应器（aerobic bioreactor）
实验设计类型术语登录号：http://purl.obolibrary.org/obo/ENVO_00002126
实验设计类型术语来源：ENVO

## 实验因子
实验因子名称：氧气、底物
实验因子类型：化学化合物（chemical compound）
实验因子类型术语登录号：http://purl.obolibrary.org/obo/OMIT_0011105、http://purl.obolibrary.org/obo/OMIT_0002057
实验因子类型术语来源：OMIT

## 实验测定项
实验测定文件名：a_Cr_5_OUR.txt、a_Cr_5_Nitrogencompounds.txt
实验测定类型：以氨为电子供体的好氧呼吸、以亚硝酸盐为电子供体的好氧呼吸；液态水中硝酸盐与亚硝酸盐浓度（原文存在笔误“electron donoraerobic”，已修正为“electron donor aerobic”）
实验测定类型术语登录号：http://purl.obolibrary.org/obo/GO_0019409、http://purl.obolibrary.org/obo/GO_0019332、http://purl.obolibrary.org/obo/ENVO_03000123
实验测定类型术语来源：ENVO
实验测定技术类型：呼吸计量法；氨氮（NH₄-N）、亚硝态氮（NO₂-N）、硝态氮（NO₃-N）检测

## 实验方案
方案名称：呼吸计量法；氨氮测定、亚硝态氮测定、硝态氮测定
方案描述：实验装置设计为污泥负荷（Food/Microorganism Ratio，F/M）比为0.024 mg NH₄⁺-N/L/mg挥发性悬浮固体（Volatile Suspended Solids，VSS），向取自实验室规模反应器的活性污泥中投加30 mg NH₄⁺-N/L的氨氮、三价铬以及宏量与微量营养元素。使用配备水套和MFCS软件的赛多利斯Biostat-B/Univessel反应器进行溶解氧测量，并计算耗氧速率（Oxygen Uptake Rate，OUR）。实验装置设计为污泥负荷比为0.024 mg NH₄⁺-N/L/mg VSS，向取自实验室规模反应器的活性污泥中投加30 mg NH₄⁺-N/L的氨氮、三价铬以及宏量与微量营养元素。在配备水套和MFCS软件的赛多利斯Biostat-B/Univessel反应器中进行氮含量检测。样品经0.22μm滤膜过滤（原文为0,22Um，欧洲格式逗号改为标准小数点）。
方案参数名称：耗氧速率（OUR）、NH₄-N、NO₂-N、NO₃-N

三价铬（Cr(III)）浓度为10 mg/L实验的原始数据。

## 本体来源参考
术语来源名称：ENVO
术语来源文件：http://purl.obolibrary.org/obo/ENVO_00002046
术语来源描述：环境本体（Environment Ontology，原文笔误为Onthology）

## 实验
实验标识：Cr(III) 10 mg/L
实验标题：三价铬影响实验
实验描述：测定投加三价铬后的硝化动力学、微生物丰度及功能特性（原文存在笔误“functioanl”，已修正为“functional”）
提交日期：2025年3月2日（原文为欧洲格式2.03.2025）

## 实验设计描述
实验设计类型：好氧生物反应器（aerobic bioreactor）
实验设计类型术语登录号：http://purl.obolibrary.org/obo/ENVO_00002126
实验设计类型术语来源：ENVO

## 实验因子
实验因子名称：氧气、底物
实验因子类型：化学化合物（chemical compound）
实验因子类型术语登录号：http://purl.obolibrary.org/obo/OMIT_0011105、http://purl.obolibrary.org/obo/OMIT_0002057
实验因子类型术语来源：OMIT

## 实验测定项
实验测定文件名：a_Cr_10_OUR.txt、a_Cr_10_Nitrogencompounds.txt
实验测定类型：以氨为电子供体的好氧呼吸、以亚硝酸盐为电子供体的好氧呼吸；液态水中硝酸盐与亚硝酸盐浓度（原文存在笔误“electron donoraerobic”，已修正为“electron donor aerobic”）
实验测定类型术语登录号：http://purl.obolibrary.org/obo/GO_0019409、http://purl.obolibrary.org/obo/GO_0019332、http://purl.obolibrary.org/obo/ENVO_03000123
实验测定类型术语来源：ENVO
实验测定技术类型：呼吸计量法；氨氮（NH₄-N）、亚硝态氮（NO₂-N）、硝态氮（NO₃-N）检测

## 实验方案
方案名称：呼吸计量法；氨氮测定、亚硝态氮测定、硝态氮测定
方案描述：实验装置设计为污泥负荷（Food/Microorganism Ratio，F/M）比为0.024 mg NH₄⁺-N/L/mg挥发性悬浮固体（Volatile Suspended Solids，VSS），向取自实验室规模反应器的活性污泥中投加30 mg NH₄⁺-N/L的氨氮、三价铬以及宏量与微量营养元素。使用配备水套和MFCS软件的赛多利斯Biostat-B/Univessel反应器进行溶解氧测量，并计算耗氧速率（Oxygen Uptake Rate，OUR）。实验装置设计为污泥负荷比为0.024 mg NH₄⁺-N/L/mg VSS，向取自实验室规模反应器的活性污泥中投加30 mg NH₄⁺-N/L的氨氮、三价铬以及宏量与微量营养元素。在配备水套和MFCS软件的赛多利斯Biostat-B/Univessel反应器中进行氮含量检测。样品经0.22μm滤膜过滤（原文为0,22Um，欧洲格式逗号改为标准小数点）。
方案参数名称：耗氧速率（OUR）、NH₄-N、NO₂-N、NO₃-N

三价铬（Cr(III)）浓度为20 mg/L实验的原始数据（原文实验标识为Cr(III) 20 mg/L，标题标注为15 mg/L实验，存在笔误）。

## 本体来源参考
术语来源名称：ENVO
术语来源文件：http://purl.obolibrary.org/obo/ENVO_00002046
术语来源描述：环境本体（Environment Ontology，原文笔误为Onthology）

## 实验
实验标识：Cr(III) 20 mg/L
实验标题：三价铬影响实验
实验描述：测定投加三价铬后的硝化动力学、微生物丰度及功能特性（原文存在笔误“functioanl”，已修正为“functional”）
提交日期：2025年3月2日（原文为欧洲格式2.03.2025）

## 实验设计描述
实验设计类型：好氧生物反应器（aerobic bioreactor）
实验设计类型术语登录号：http://purl.obolibrary.org/obo/ENVO_00002126
实验设计类型术语来源：ENVO

## 实验因子
实验因子名称：氧气、底物
实验因子类型：化学化合物（chemical compound）
实验因子类型术语登录号：http://purl.obolibrary.org/obo/OMIT_0011105、http://purl.obolibrary.org/obo/OMIT_0002057
实验因子类型术语来源：OMIT

## 实验测定项
实验测定文件名：a_Cr20_OUR.txt、a_Cr_20_Nitrogencompounds.txt
实验测定类型：以氨为电子供体的好氧呼吸、以亚硝酸盐为电子供体的好氧呼吸；液态水中硝酸盐与亚硝酸盐浓度（原文存在笔误“electron donoraerobic”，已修正为“electron donor aerobic”）
实验测定类型术语登录号：http://purl.obolibrary.org/obo/GO_0019409、http://purl.obolibrary.org/obo/GO_0019332、http://purl.obolibrary.org/obo/ENVO_03000123
实验测定类型术语来源：ENVO
实验测定技术类型：呼吸计量法；氨氮（NH₄-N）、亚硝态氮（NO₂-N）、硝态氮（NO₃-N）检测

## 实验方案
方案名称：呼吸计量法；氨氮测定、亚硝态氮测定、硝态氮测定
方案描述：实验装置设计为污泥负荷（Food/Microorganism Ratio，F/M）比为0.024 mg NH₄⁺-N/L/mg挥发性悬浮固体（Volatile Suspended Solids，VSS），向取自实验室规模反应器的活性污泥中投加30 mg NH₄⁺-N/L的氨氮、三价铬以及宏量与微量营养元素。使用配备水套和MFCS软件的赛多利斯Biostat-B/Univessel反应器进行溶解氧测量，并计算耗氧速率（Oxygen Uptake Rate，OUR）。实验装置设计为污泥负荷比为0.024 mg NH₄⁺-N/L/mg VSS，向取自实验室规模反应器的活性污泥中投加30 mg NH₄⁺-N/L的氨氮、三价铬以及宏量与微量营养元素。在配备水套和MFCS软件的赛多利斯Biostat-B/Univessel反应器中进行氮含量检测。样品经0.22μm滤膜过滤（原文为0,22Um，欧洲格式逗号改为标准小数点）。
方案参数名称：耗氧速率（OUR）、NH₄-N、NO₂-N、NO₃-N