The subgingival microbiota communities in periodontal health and periodontitis studied by sequencing the V1-V3 16S rRNA gene region.

The present bioproject contains 118 subgingival plaque samples obtained from subjects with different periodontal health conditions, which were analysed by amplifying and sequencing the V1-V3 16S rRNA gene region using an Illumina MiSeq instrument.The experiment consisted of 53 periodontally healthy and 65 periodontitis patients. Among the latter, 6 were classified as moderate and 59 as severe. The periodontitis cases were diagnosed according to the classification of Page and Eke (J Periodontol. 2007; 78:1387-99) The attached metadata table contains information regarding the following variables for each participant: health condition, health of the sampling sites, periodontitis type (if applicable), periodontitis severity (if applicable), sample type, paired design, therapy, sample time after the diagnosis, age, sex, ethnicity, systemic condition, smoking habit (including number of cigarettes/day and months smoking), and clinical parameters (total number of teeth, plaque index, bleeding on probing, probing pocket depth (PPD) and clinical attachment level (CAL); and PPD and CAL of the sampling sites.The study subjects were aged between 24 and 75 years old, 65 of them were females and 53 males, and there were 74 non-smokers and 44 smokers. All individuals were systemically healthy and Caucasian.Also 6 mock samples named HM276, HM277, and D6303, were included to be used as quality control of sequences

本生物项目共包含118份龈下菌斑样本，采集自不同牙周健康状态的受试者。本研究通过Illumina MiSeq仪器对V1-V3区16S rRNA基因片段进行扩增并测序，完成所有样本的分析。本次实验共纳入53名牙周健康者与65名牙周炎患者；其中6例为中度牙周炎，59例为重度牙周炎。牙周炎病例的诊断依据Page与Eke于2007年发表于《Journal of Periodontology》的分类标准（J Periodontol. 2007; 78:1387-99）。随附的元数据表包含每名受试者的如下变量信息：健康状态、采样位点健康状况、牙周炎类型（如适用）、牙周炎严重程度（如适用）、样本类型、配对实验设计、治疗方案、诊断后采样时间、年龄、性别、种族、全身健康状况、吸烟习惯（含每日吸烟支数与吸烟时长），以及临床参数：总牙数、菌斑指数、探诊出血情况、探诊深度（PPD）、临床附着丧失（CAL），以及采样位点的PPD与CAL。本研究受试者年龄介于24至75岁，其中女性65名，男性53名；非吸烟者74名，吸烟者44名。所有受试者均无系统性基础疾病，且为高加索人种。此外本研究还纳入HM276、HM277与D6303共6份模拟样本，用作序列测序的质量质控对照。