Gene expression comparison of exhausted CD8 T cells after PD-L1 blockade alone or PD-L1 blockade combined with LPS. Gene expression comparison of exhausted CD8 T cells after PD-L1 blockade alone or PD-L1 blockade combined with LPS

CD8 T cells after combined treatment exhibit a distinct molecular signature Overall design: We FACS-sorted exhausted CD8 T cells from chronically infected mice (lifelong model of LCMV Cl-13 infection) given PD-L1 antibody alone or PD-L1 antibody with LPS

联合治疗后的CD8 T细胞（CD8 T cells）呈现独特的分子特征。实验整体设计：本研究通过荧光激活细胞分选术（Fluorescence-activated cell sorting, FACS），从淋巴细胞脉络丛脑膜炎病毒Cl-13（Lymphocytic choriomeningitis virus Cl-13, LCMV Cl-13）终身感染模型的慢性感染小鼠体内分选出耗竭性CD8 T细胞（exhausted CD8 T cells）；所有受试小鼠分别接受单一PD-L1抗体（PD-L1 antibody）治疗，或PD-L1抗体联合脂多糖（Lipopolysaccharide, LPS）治疗。