Aditional file 2.

Gametocyte density of P. vivax cultured with different sources of reticulocytes in 7 days. Reticulocytes purified from peripheral blood (red line), cord blood (blue line) and hematopoietic stem cell (green line) were used to cultured fresh isolates of P. vivax (11 isolates). Reticulocytes were added to the cultures daily at a final 4% and the gametocyte density was determined for 7 days from Giemsa-stained thick smears (1 Âľl packed cells). Each line represents average parasite density from 7 days obtained from 11 P. vivax isolates. Cord blood-reticulocytes has highly expression of fetal hemoglobin but still shown better support gametocyte production in some parasite isolates (VKTS33, VKBT59, VKBT63, and VKBT81).

本数据集记录了7天体外培养周期内，不同来源网织红细胞培养间日疟原虫（Plasmodium vivax）的配子体密度。实验使用从外周血（红线）、脐带血（蓝线）及造血干细胞（绿线）中纯化得到的网织红细胞，对11株间日疟原虫新鲜临床分离株进行培养。每日向培养体系中添加终浓度为4%的网织红细胞，通过吉姆萨染色厚血膜（每样品取1 μL压积细胞）计数，测定7天培养周期内的配子体密度。每条曲线代表11株间日疟原虫分离株7天培养所得的寄生虫密度平均值。脐带血来源的网织红细胞虽高表达胎儿血红蛋白，但在部分疟原虫分离株（VKTS33、VKBT59、VKBT63及VKBT81）中仍展现出更优异的配子体生成支持能力。