blastn_RAD_datasets_11_20_07022024B.tar

16S and 23S ribosomal RNA gene fragments sequenced by a MinION nanopore system were used to obtain sub-species resolution of datasets from a D6322 mock bacterial microbiome. Correct strain resolution usually resulted over a 3 order of magnitude variation in species proportions.

本研究通过MinION纳米孔测序系统（MinION nanopore system）对16S与23S核糖体RNA（ribosomal RNA, rRNA）基因片段进行测序，以此获取D6322模拟细菌微生物组（D6322 mock bacterial microbiome）数据集的亚种分辨率（sub-species resolution）。当实现准确的菌株分辨率（strain resolution）时，通常可观测到物种占比存在超过3个数量级的差异。