Genomic alteration analysis in ovarian cancers

In order to check if there is a correlation between centrosome alterations and DNA breakpoints or homologous recombination defects (HRD) assesssed by LST genomic signatures, we used Affimetrix cytoscan array and GAP tool as previously described in Goundiam et al, IJC 2015 (PMID:25892415 DOI:10.1002/ijc.29568 ) 67 high grade serous ovarian cancers For these samples we checked if there is a correlation between DNA breakpoints or chromosome content (from cytoscan array) and the number of centrosomes defined by immunoflurescence

为探究中心体异常与DNA断裂点，或经LST基因组特征（LST genomic signatures）评估的同源重组缺陷（homologous recombination defects, HRD）之间是否存在相关性，本研究采用Affimetrix细胞扫描芯片（Affimetrix cytoscan array）与GAP工具（GAP tool），实验方案参照Goundiam等于2015年发表于《国际癌症杂志》（IJC）的研究（PMID:25892415，DOI:10.1002/ijc.29568），共纳入67例高级别浆液性卵巢癌（high grade serous ovarian cancers）样本。针对上述样本，我们进一步分析了DNA断裂点或染色体组成（源自细胞扫描芯片检测结果）与免疫荧光（immunofluorescence）染色定量的中心体数量之间的相关性。