Airway metagenome of children in Kilifi, Kenya

Naso- and oropharyngeal samples were collected from children with different manifestations of respiratory infection as well as from well controls. Upper airway microbiota were characterised by sequencing the V3/V4 hypervariable region of the bacterial 16S rRNA gene on the Illumina MiSeq platform.

本研究从表现出不同呼吸道感染表型的儿童及健康对照个体中采集了鼻与口咽样本。随后通过在Illumina MiSeq测序平台上对细菌16S rRNA基因（16S rRNA gene）的V3/V4高变区进行测序，对受检样本的上呼吸道微生物群完成了特征表征与分析。