Data Sheet 1_Ectophoma salviniae sp. nov., Neottiosporina mihintaleensis sp. nov. and four other endophytes associated with aquatic plants from Sri Lanka and their extracellular enzymatic potential.pdf

Endophytic fungi associated with selected aquatic plants, Eichhornia crassipes, Nymphaea nouchali, Salvinia minima and S. molesta were evaluated. Ectophoma salviniae sp. nov. and Neottiosporina mihintaleensis sp. nov. are introduced as novel taxa from Salvinia spp. from Sri Lanka. Chaetomella raphigera is reported as a new geographical record, Colletotrichum siamense and C. truncatum are reported as novel host records in aquatic plants, while Phyllosticta capitalensis has been identified on the same host (Nymphaea nouchali) in the North-Central Province of Sri Lanka. Identification of the fungi was based on morphological characteristics and multi-locus phylogenetic analyses using ITS, LSU, SSU, ACT, CHS-1, GAPDH, tub2, rpb2, and tef1-α molecular markers. The identified fungi were analysed for extracellular enzymatic properties. According to the qualitative analysis, Ectophoma salviniae sp. nov. exhibited the highest amylase production, Chaetomella raphigera exhibited the highest cellulase enzyme production, and Neottiosporina mihintaleensis sp. nov. exhibited the highest laccase production. The results demonstrate the aquatic fungal diversity in this region and their extracellular enzymatic potentials, providing valuable insights for future biotechnological approaches.

本研究对选取的水生植物——凤眼莲（Eichhornia crassipes）、子午莲（Nymphaea nouchali）、小槐叶萍（Salvinia minima）及巨槐叶萍（S. molesta）所关联的内生真菌开展了系统评估。从斯里兰卡的槐叶萍属（Salvinia spp.）植物中，报道了两个全新的分类单元：槐叶萍外囊壳霉新种（Ectophoma salviniae sp. nov.）与米欣塔利类无丝孢霉新种（Neottiosporina mihintaleensis sp. nov.）。刚毛壳霉（Chaetomella raphigera）被记录为斯里兰卡境内的新地理分布物种；暹罗炭疽菌（Colletotrichum siamense）与截断炭疽菌（C. truncatum）则为水生植物上的新寄主记录；此外，本研究在斯里兰卡北中省的子午莲（Nymphaea nouchali）上首次鉴定出资本叶点霉（Phyllosticta capitalensis）。所有供试真菌的鉴定均基于形态学特征，以及利用内转录间隔区（ITS）、大亚基核糖体RNA（LSU）、小亚基核糖体RNA（SSU）、肌动蛋白基因（ACT）、几丁质合酶1基因（CHS-1）、甘油醛-3-磷酸脱氢酶基因（GAPDH）、β-微管蛋白基因（tub2）、RNA聚合酶II第二大亚基基因（rpb2）与翻译延伸因子1-α基因（tef1-α）共9个分子标记开展的多基因座系统发育分析。本研究同时对所鉴定的真菌进行了胞外酶特性检测。定性分析结果显示，槐叶萍外囊壳霉新种的淀粉酶产量最高，刚毛壳霉的纤维素酶合成能力最强，米欣塔利类无丝孢霉新种的漆酶产量最优。本研究结果阐明了该区域的水生真菌多样性及其胞外酶应用潜力，为未来生物技术相关研究方向提供了极具价值的参考依据。