Physiological response of goldsinny wrasse to global climate change

In order to understand the physiological effects of global climate change on goldsinny wrasse, a multi-stressor experiment was conducted exposing the fish to four weeks of experimental conditions with treatments consisting of control conditions (salinity approx 28, temp 13°C, pH approx 8.1), low salinity (17), high temperature (19°C), low ph (7.5) and the combination of all three stressors combined (multi-stressor treatment salinity 17, temp 19°C, and pH 7.5).
Potential physiological stress was evaluated by measuring growth (weight and length), oxygen consumption using respirometry, and liver oxidative stress analyses (ratio GSSG-GSH, GSSG, GSH, CAT, GST, GR and LPO).

Data from the Weight Length file contains information related to experimental treatment conditions, fish ID, and weight (g) and length (cm) at the end of the exposure period (4 columns, 123 rows).

Data from the ratio GSSG-GSH, GSSG, GSH, CAT, GST, GR and LPO files are from enzymatic measurements of liver samples for oxidative stress analyses and include treatment and fish ID for each biomarker.

The ratio GSSG-GSH file - 3 columns, 80 rows; the GSSG file – 3 columns, 80 rows; the GSH file – 3 columns, 80 rows; the CAT file - 3 columns, 74 rows; the GST file – 3 columns, 77 rows; the GR file – 3 columns, 75 rows; the LPO file – 3 columns, 77 rows. Glutathione-reductase = GR (nmol/mg protein/min), Glutathione-S-transferase = GST (µmol/mg protein/min), Catalase = CAT (µmol/mg protein/min), Reduced glutathione = GSH (µmol/mg liver), Oxidized glutathione = GSSG (µmol/mg liver), Ratio oxidized/reduced = %GSSG/GSH (%), Lipid peroxidation = LPO (nmol MDA/ml).

Data from the Rest, max and scope respirometry file contains oxygen consumption data MO2 which stands for mass specific O2 uptake and SMR for standard metabolic rate. SMR, maximum MO2 and aerobic scope (mg O2 h-1). It also includes treatment, fish ID, and weight (g) – 6 columns, 76 rows.

为探究全球气候变化对岩金隆头鱼（goldsinny wrasse）的生理影响，本研究开展多胁迫因子实验，将受试鱼类暴露于五种实验条件中，持续4周：对照组（盐度约28、温度13℃、pH约8.1）、低盐胁迫组（盐度17）、高温胁迫组（19℃）、低pH胁迫组（7.5），以及三者联合胁迫组（盐度17、温度19℃、pH7.5）。本研究通过测量生长指标（体重与体长）、采用呼吸代谢法测定耗氧量，以及开展肝脏氧化应激分析（包括GSSG-GSH比值、GSSG、GSH、CAT、GST、GR与LPO），评估潜在的生理胁迫水平。体重与体长文件包含实验处理条件、鱼只编号，以及暴露周期结束时的体重（单位：g）与体长（单位：cm）数据，共4列，123行。GSSG-GSH比值、GSSG、GSH、CAT、GST、GR与LPO文件的数据均来自肝脏样本的酶学测定，用于氧化应激分析，包含各生物标志物对应的处理组与鱼只编号。各文件的行列数如下：GSSG-GSH比值文件：3列，80行；GSSG文件：3列，80行；GSH文件：3列，80行；CAT文件：3列，74行；GST文件：3列，77行；GR文件：3列，75行；LPO文件：3列，77行。各指标的定义为：谷胱甘肽还原酶（GR，nmol/mg蛋白/min）、谷胱甘肽S-转移酶（GST，µmol/mg蛋白/min）、过氧化氢酶（CAT，µmol/mg蛋白/min）、还原型谷胱甘肽（GSH，µmol/mg肝组织）、氧化型谷胱甘肽（GSSG，µmol/mg肝组织）、氧化型/还原型比值（%GSSG/GSH，%）、脂质过氧化（LPO，nmol MDA/ml，即nmol丙二醛/ml）。静息、最大耗氧量与有氧代谢范围呼吸代谢文件包含耗氧量数据MO2（即质量特异性氧摄取率）与SMR（即标准代谢率），具体指标包括SMR、最大MO2与有氧代谢范围（单位：mg O2 h-1），同时包含处理组、鱼只编号与体重（单位：g），共6列，76行。