Picornavirus-like particle production in plants

A method of producing a picornavirus-like particle (PVLP) in a plant is provided. The method comprises introducing a first nucleic acid and a second nucleic acid into the plant， portion of the plant， or a plant cell. The first nucleic acid comprising a first regulatory region active in the plant operatively linked to a nucleotide sequence encoding a polyprotein. The second nucleic acid comprises a second regulatory region active in the plant and operatively linked to a nucleotide sequence encoding one or more protease. The plant， portion of the plant， or plant cell is incubated under conditions that permit the expression of the nucleic acids， thereby producing the PVLP. A PVLP comprising the polyprotein is also provided.

本发明提供了一种在植物中生产类小核糖核酸病毒颗粒（picornavirus-like particle，PVLP）的方法。该方法包括将第一核酸与第二核酸导入植物、植物的部分组织或植物细胞中。所述第一核酸包含在植物中具有活性的第一调控区域，该调控区域与编码多聚蛋白的核苷酸序列可操作地连接；所述第二核酸包含在植物中具有活性的第二调控区域，该调控区域与编码一种或多种蛋白酶的核苷酸序列可操作地连接。将所述植物、植物的部分组织或植物细胞置于允许核酸表达的条件下培养，即可得到所述PVLP。本发明还提供了一种包含该多聚蛋白的PVLP。