Gene expression data from MCF-7 cells treated with Lacciac Acid A

Lacciac Acid A was indentified as an inhibitor of DMNT1. MCF-7 cells were treated with Lacciac Acid A (200 uM) for 5 days. Changes in gene expression were identified by using Affymetrix Human gene ST1.0 arrays. We used microarrays to determine global changes in gene expression upon treatment with Lacciac Acid A an inhibitor of DMNT1. MCF-7 cells near mid passage were treated with Lacciac Acid A for 5 days. RNA was extracted and hybridized on Affymetrix microarrays. Treated and untreated cells were from the same cell passage. Two sample sets from the same passage and the third set from the previous passage were used.

Lacciac酸A（Lacciac Acid A）被鉴定为DNA甲基转移酶1（DMNT1）的抑制剂。将MCF-7细胞以200 μM的Lacciac酸A处理5天，通过Affymetrix人类基因ST1.0芯片（Affymetrix Human Gene ST1.0 Arrays）检测基因表达变化。本研究利用基因芯片技术，分析经DNA甲基转移酶1（DMNT1）抑制剂Lacciac酸A处理后的细胞全局基因表达变化。取处于传代中期的MCF-7细胞，以Lacciac酸A处理5天，提取细胞总RNA并在Affymetrix基因芯片上完成杂交。处理组与对照组细胞取自同一传代批次。本次实验共使用三组样本：其中两组样本来自同一传代批次，第三组样本则取自前一代次的细胞。