HEK293 cells overexpressing wild type HNF4a2 or mutant forms HNF4a2 C106R or HNF4a2 R154X. Homo sapiens

Analysis of gene expression in human embryonic kidney cells HEK293 overexpressing the transcription factors HNF4a2 or mutant forms HNF4a2 C106R and HNF4a2 R154X, which were introduced by FRT/FLP recombination. The analysis was performed 24 hours following 1µg/ml tetracycline treatment to induce the expression of the genes. Results identify HNF4a regulated genes in kidney cells being several of these genes deregulated in renal cell carcinoma. Keywords = HEK293 embryonic kidney cells Keywords = HNF4a2 Keywords = tetracycline Keywords = FRT Keywords: ordered

本研究针对过表达转录因子HNF4a2或其突变体HNF4a2 C106R、HNF4a2 R154X的人胚肾细胞HEK293（human embryonic kidney cells HEK293）展开基因表达分析，上述转录因子通过FRT/FLP重组（FRT/FLP recombination）系统导入细胞。实验采用1微克/毫升四环素（tetracycline）诱导靶基因表达，于诱导24小时后完成检测分析。研究结果鉴定出肾细胞中受HNF4a2调控的基因，其中多个基因在肾细胞癌中存在表达失调现象。关键词：HEK293人胚肾细胞、HNF4a2、四环素、FRT、有序