RNA-seq at different days during the conversion of mouse fibroblasts into corneal endothelia by defined small molecules

we established a stepwise, small-molecule-based method to reprogram mouse fibroblasts into expandable chemically induced neural crest cells (ciNCCs) and their derivative, corneal endothelial cells (ciCECs). Overall design: RNA expression at D0, D7, D12, ciCECs,primary CECs

本研究建立了一种基于小分子的逐步重编程策略，可将小鼠成纤维细胞重编程为可扩增的化学诱导神经嵴细胞（ciNCCs）及其衍生的角膜内皮细胞（ciCECs）。实验整体设计：对第0天（D0）、第7天（D7）、第12天（D12）、ciCECs以及原代角膜内皮细胞（primary CECs）的RNA表达水平进行检测。