Expression data from cultured human esophageal squamous cell carcinoma cell lines and cultured human fibroblasts.. Homo sapiens

Cancer cells express different sets of receptor type tyrosine kinases. These receptor kinases may be activated through autocrine or paracrine mechanisms. Fibroblasts may modify the biologic properties of surrounding cancer cells through paracrine mechansms. We used microarrays to obtain the global gene expression of human esophageal squamous cell carcinoma cell lines and various esophageal fibroblasts. Overall design: A total of 22 human esophageal squamous cell carcinoma cell lines and fibroblasts were analyzed. 4 fibroblasts were obtained from surgically resected human esophagus. Cells were cultured under standard conditions, total RNA was extracted and hybridized on Affymetrix microarrays.

癌细胞表达不同组合的受体型酪氨酸激酶（receptor type tyrosine kinases）。此类受体激酶可通过自分泌或旁分泌机制被激活。成纤维细胞可通过旁分泌机制改变周围癌细胞的生物学特性。本研究采用微阵列（microarrays）技术获取人食管鳞状细胞癌细胞系及不同食管成纤维细胞的全基因组基因表达谱。实验设计概述：共计分析22株人食管鳞状细胞癌细胞系及成纤维细胞，其中4株成纤维细胞取自手术切除的人食管组织。所有细胞均在标准培养条件下培养，随后提取总RNA，并在Affymetrix微阵列芯片上进行杂交实验。