Comparison of the human oocyte to its sister polar body

Clinicians need additional metrics for predicting quality of human oocytes for IVF procedures. Human polar bodies reflect the oocyte transcript profile. Quantitation of polar body mRNAs could allow for both oocyte ranking and embryo preferences in IVF applications. The transcriptome of a polar body has never been reported, in any organism. Eight total samples. There are 2 biological replicates of the following four conditions: pooled oocytes and their sister polar bodies and a single oocyte and its sister polar body.

临床医师亟需更多评估指标，以预测用于体外受精（In Vitro Fertilization，IVF）操作的人类卵母细胞（oocyte）质量。人类极体（polar body）可反映卵母细胞的转录谱（transcript profile）。对极体信使RNA（messenger RNA，mRNA）进行定量分析，可在IVF应用中实现卵母细胞分级筛选与胚胎优选。目前尚无任何物种的极体转录组（transcriptome）相关研究报道。本数据集共包含8个样本，以下4种实验条件各设置2次生物学重复（biological replicates）：合并收集的卵母细胞、合并卵母细胞的同源极体、单个卵母细胞以及单个卵母细胞的同源极体。