Human ES cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage. Homo sapiens

All data are derived from the same batch of human ES cells (line H9, WA-09). With the exception of the “UD” file all files represent neural cell types derived from the undifferentiated hESCs. Following mechanical isolation, rosettes were maintained for various passages in the presence of defined growth factors and morphogen factors as indicated below at the R-NSC stage. Cells at the NSCFGF/EGF stage were derived from mechanically isolated neural rosettes that were purified and long-term expanded as attached monolayer cultures in serum free medium in the presence of FGF2/EGF. Keywords: differentiation of human ES cells (line H9, WA-09). Overall design: All data are derived from the same batch of human ES cells (line H9, WA-09). With the exception of the “UD” file all files represent neural cell types derived from the undifferentiated hESCs. Following mechanical isolation, rosettes were maintained for various passages in the presence of defined growth factors and morphogen factors as indicated below at the R-NSC stage. Cells at the NSCFGF/EGF stage were derived from mechanically isolated neural rosettes that were purified and long-term expanded as attached monolayer cultures in serum free medium in the presence of FGF2/EGF.

所有数据均来源于同一批次的人类胚胎干细胞（human ES cells）H9（WA-09）细胞系。除"UD"文件外，其余所有文件均代表源自未分化人类胚胎干细胞的神经细胞类型。经机械分离后，神经玫瑰样结构（neural rosettes）在R-NSC阶段于下述指定的限定性生长因子及形态发生因子存在条件下进行了多次传代培养。NSC FGF/EGF阶段的细胞则源自经机械分离得到的神经玫瑰样结构，该结构经纯化后，在添加FGF2/EGF的无血清培养基中以贴壁单层培养的方式进行长期扩增。关键词：人类胚胎干细胞（H9，WA-09细胞系）的分化。整体实验设计：所有数据均来源于同一批次的人类胚胎干细胞（human ES cells）H9（WA-09）细胞系。除"UD"文件外，其余所有文件均代表源自未分化人类胚胎干细胞的神经细胞类型。经机械分离后，神经玫瑰样结构（neural rosettes）在R-NSC阶段于下述指定的限定性生长因子及形态发生因子存在条件下进行了多次传代培养。NSC FGF/EGF阶段的细胞则源自经机械分离得到的神经玫瑰样结构，该结构经纯化后，在添加FGF2/EGF的无血清培养基中以贴壁单层培养的方式进行长期扩增。