The de novo DNA methyltransferase DNMT3C protects male fertility from transposon activity (RNA-Seq).

To allow identification of differentially expressed genes and transposons in Dnmt3C IAP/IAP mutants, mutant and control RNA-Seq experiments were conducted starting from whole testis RNA. Overall design: Comparison of RNA-seq results from whole testis RNA of mutant Dnmt3C IAP/IAP and control Dnmt3C IAP/WT at 16.5 dpc, 10 dpp, and 20 dpp.

为鉴定Dnmt3C IAP/IAP突变体中的差异表达基因与转座子，本研究以完整睾丸RNA为起始材料，开展了突变体与对照组的RNA测序（RNA-Seq）实验。
实验整体设计：分别于妊娠16.5天（days post coitum, dpc）、出生后10天（days post partum, dpp）与出生后20天（dpp）三个时间点，对比分析Dnmt3C IAP/IAP突变体与Dnmt3C IAP/WT对照组的完整睾丸RNA测序结果。