Effect of IVIG on the activation of plasmacytoid dendritic cells

To investigate the effect of IVIG and desialylated IVIG on the activation of plasmacytoid dendritic cells (pDCs). Human primary plasmacytoid dendritic cells were treated with TLR stimulation together with or without IVIG or desialylated IVIG, and the change of genes were analyzed. Primary human pDCs were preincubated with or without 10mg/ml IVIG or desialylated IVIG followed by stimulation with CpG overnight. The different genes between IVIG+CpG and desialylated IVIG+CpG were analyzed. Overall design: Comparative gene expression profiling analysis of RNA-seq data for pDCs with different treatment. Three biological replicates were made for each treatment. The reference samples are CpG stimulated pDCs.

本研究旨在探究静脉注射免疫球蛋白（IVIG）与去唾液酸化静脉注射免疫球蛋白（desialylated IVIG）对浆细胞样树突状细胞（plasmacytoid dendritic cells, pDCs）活化的影响。实验中，将人原代浆细胞样树突状细胞经Toll样受体（Toll-like receptor, TLR）刺激，并联合或不联合IVIG、去唾液酸化IVIG处理，随后对其基因表达变化进行分析。
具体预处理步骤为：将人原代pDCs以10mg/ml浓度的IVIG或去唾液酸化IVIG预处理（或不进行预处理），随后使用CpG刺激并过夜培养。后续对IVIG联合CpG组与去唾液酸化IVIG联合CpG组之间的差异表达基因进行分析。
实验设计概况：对不同处理组pDCs的RNA测序（RNA-seq）数据开展比较基因表达谱分析。每个处理组设置3次生物学重复，参照样本为经CpG刺激的pDCs。