Transcripts induced by exposure to SLE serum in healthy blood monocytes

Monocytes from 3 healthy donors were cultured for 6 hours in the presence of 20% serum from three newly diagnosed, untreated SLE patients. Microarray analysis was then performed upon normalizing the gene expression levels of samples incubated with SLE sera to those incubated with autologous serum. Monocytes from 3 healthy donors were cultured for 6 hours in the presence of 20% serum from three newly diagnosed, untreated SLE patients. Microarray analysis was then performed upon normalizing the gene expression levels of samples incubated with SLE sera to those incubated with autologous serum.

选取3名健康供者的单核细胞，在3名新诊断且未接受治疗的系统性红斑狼疮（Systemic Lupus Erythematosus, SLE）患者的20%血清中培养6小时。随后将经SLE血清孵育的样本的基因表达水平标准化至经自体血清孵育的样本的基因表达水平后，进行微阵列分析。
选取3名健康供者的单核细胞，在3名新诊断且未接受治疗的系统性红斑狼疮（Systemic Lupus Erythematosus, SLE）患者的20%血清中培养6小时。随后将经SLE血清孵育的样本的基因表达水平标准化至经自体血清孵育的样本的基因表达水平后，进行微阵列分析。