May et al_PBIOLOGY-D-24-01652R2_FACS

The purpose of the experiment was to isolate fluorescently tagged cells from single cell suspensions of <i>Drosophila</i> <i>melanogaster</i><i> </i>embryos aged to stage 10-12. Cells were expressing a fluorescently tagged protein localising to the plasma membrane in either the salivary gland primordium only (SrcGFP) or in all of the embryonic epithelium (ArmYFP). The isolated fluorescent cells were sent for single cell RNA sequencing to investigate the genetic programmes involved in early salivary gland tubulogenesis.

本实验旨在从发育至10-12期的黑腹果蝇（Drosophila melanogaster）胚胎的单细胞悬液中分离荧光标记细胞。所用细胞分别表达定位于质膜的荧光标记蛋白：一类仅在唾液腺原基中表达（SrcGFP），另一类在全部胚胎上皮中表达（ArmYFP）。将分离得到的荧光细胞进行单细胞RNA测序，以探究参与早期唾液腺管发生的遗传程序。