Gene expression data from MGH-U3, RT112 and UM-UC-5 following TYRO3 knock-down

To better understand the molecular mechanisms underlying TYRO3 oncogenic activity in bladder carcinomas, we made use of MGH-U3, RT112 and UM-UC-5 cell lines, which were derived from a human bladder tumor and endogenously expressed the Tyro3 protein, the growth and transformation of these cell lines being dependent on Tyro3. We carried out a gene expression analysis using Affymetrix DNA arrays in this cell line treated or not withTYRO3 siRNAs. MGH-U3, RT112 and UM-UC-5 cells were transfected or not with siRNA TYRO3. mRNA was extracted after 40h and gene expression analysis was assessed using the HG-U133 Plus 2.0 array (Affymetrix) array

为深入解析膀胱癌中TYRO3致癌活性的分子机制，我们选用了MGH-U3、RT112及UM-UC-5三株细胞系。上述细胞系均来源于人类膀胱肿瘤，且可内源性表达Tyro3蛋白，其生长与转化过程均依赖于Tyro3。我们采用Affymetrix DNA芯片开展基因表达分析，对经或未经TYRO3小干扰RNA（small interfering RNA, siRNA）处理的上述细胞系进行检测。具体操作如下：将MGH-U3、RT112及UM-UC-5细胞分别转染TYRO3 siRNA或设置空白对照（未转染），于转染40小时后提取mRNA，并使用HG-U133 Plus 2.0基因芯片（Affymetrix）完成基因表达检测。