Supplemental Tables S4&S12

<i>Dendrobium catenatum</i> is an orchid herb used in traditional Chinese medicine with high pharmacological and nutritional value. <i>D. catenatum</i> obtained by simulated wild cultivation is highly favored due to its high quality. However, southern blight disease caused by<i> Sclerotium delphinii</i> leads to devastating losses in simulated wild cultivation of <i>D. catenatum</i>. Previously, we showed that the core jasmonic acid (JA) signaling factor DcMYC2b positively regulates plant defense against southern blight disease. We found that the MYC2 mode of action in plant defense is species-specific, but the underlying regulatory mechanism of DcMYC2b is unclear. Here, a comprehensive analysis involving weighted gene coexpression network analysis (WGCNA), metabolomics, and transcription factor -centered yeast one-hybrid (TF-centered Y1H) assays was performed to elucidate the crosstalk between the core JA signaling and phenylpropanoid pathways, as well as the molecular mechanisms underlying the defense of <i>D. catenatum</i> against southern blight disease.

铁皮石斛（Dendrobium catenatum）是一种兰科草本药用植物，在传统中医药中应用广泛，兼具极高的药理与营养价值。通过仿野生栽培方式获得的铁皮石斛因品质优异而深受市场青睐。然而，由德尔夫林小核菌（Sclerotium delphinii）引发的南方白绢病，给铁皮石斛的仿野生栽培产业带来了毁灭性损失。本课题组此前的研究证实，核心茉莉酸（JA）信号通路因子DcMYC2b可正向调控植株对南方白绢病的防御反应。我们发现，MYC2在植物防御中的作用模式具有物种特异性，但DcMYC2b的具体调控机制仍不明晰。本研究通过整合加权基因共表达网络分析（weighted gene coexpression network analysis, WGCNA）、代谢组学以及以转录因子为中心的酵母单杂交（TF-centered Y1H）实验，系统解析了核心JA信号通路与苯丙烷代谢途径之间的交叉调控关系，以及铁皮石斛抵御南方白绢病的分子机制。