Mannanase activity of selected Y. lipolytica variants.

The mannanase activity was measured at 40°C in sodium acetate buffer 50 mM, pH 5.2 using 1% (w/v) galactomannan. The coefficient of variation (CV) was defined as the ratio of the standard deviation to the mean and was calculated for each of the wild-type enzymes. wt, wild type.本实验于40℃、50 mM乙酸钠缓冲液（pH 5.2）中，以1%（w/v）半乳甘露聚糖为底物，测定甘露聚糖酶活性。变异系数（coefficient of variation, CV）定义为标准差与均值的比值，针对每一株野生型酶分别计算。wt即野生型（wild type）。