NCB-A50345-T_Sequencing and mass spectrum raw data_Chen et al.

Sequencing data: Next-generation sequencing data is from the PARPi resistance CRISPR screen describing in this paper, by analyzing the sgRNA abundance of GeCKO v2 CRISPR knockout libraries A and B at the indicated time points in the cells before and after olaparib treatment. Including <strong>library-A-0d</strong> / <strong>library-B-0d</strong> / <strong>library-A-30d</strong> / <strong>library-B-30d and a MD5 file.</strong> <br> Mass spectrum data: For mass spectrometry, on-bead trypsin digestion was performed and liquid chromatography-tandem MS (LC-MS/MS) was carried out on the Thermo Q Exactive Hybrid quadrupole orbitrap mass spectrometer as described in this paper. Including <strong>BioID-NLS-1</strong> / <strong>BioID-NLS-2 /</strong> <strong>BioID-NUMEN / NUMEN-BioID.</strong>

测序数据：本文报道的PARP抑制剂（PARPi）耐药CRISPR筛选实验所产生的下一代测序（Next-generation sequencing）数据，通过分析奥拉帕利（olaparib）处理前后细胞中GeCKO v2 CRISPR敲除文库A与B的单导RNA（sgRNA）丰度，获取指定时间点的样本数据。数据集包含<strong>library-A-0d</strong> / <strong>library-B-0d</strong> / <strong>library-A-30d</strong> / <strong>library-B-30d</strong> 以及一个MD5校验文件。<br>质谱数据：实验采用磁珠上胰蛋白酶酶解方法，并按照本文所述流程，在赛默飞Q Exactive混合型四极杆-轨道阱质谱仪上完成液相色谱-串联质谱（liquid chromatography-tandem MS, LC-MS/MS）检测。数据集包含<strong>BioID-NLS-1</strong> / <strong>BioID-NLS-2</strong> / <strong>BioID-NUMEN</strong> / <strong>NUMEN-BioID</strong>。