Peptide library purity by mass spectrometry (Coassolo et al., 2023)

The library was produced as crude peptides at a quantity of 1-4mg with a C-terminal amidation as the only modification for comparative studies between peptides. Identity and purity of the peptides used in vivo were verified by LC-MS using an Agilent Q-TOF LC–MS instrument. The raw mass spectrometry (MS1 spectra) data are deposited as Agilent .d file for the six synthesized peptides used in the in vivo screen (BRINP2_peptide 5.d, EDIL3_peptide 5.d, FGF3_peptide 4.d, FGF5_peptide 5.d, FSTL4_peptide 3.d and SCG1_peptide 9.d).

本数据集所包含的肽库以粗肽形式制备，单批次产量为1~4mg，仅采用C端酰胺化作为唯一修饰方式，用于开展肽段间的对比研究。本研究体内实验所用肽段的身份与纯度，通过安捷伦（Agilent）Q-TOF液相色谱-质谱联用（LC-MS）仪器完成验证。本研究体内筛选所用的6条合成肽段的原始质谱（MS1谱图）数据，以安捷伦.d格式文件存档，具体文件包括：BRINP2_peptide 5.d、EDIL3_peptide 5.d、FGF3_peptide 4.d、FGF5_peptide 5.d、FSTL4_peptide 3.d与SCG1_peptide 9.d。