METTL14-mediated N6-methyladenosine modification of Col17a1/Itgα6/Itgβ4 governs epidermal homeostasis [MeRIP-seq]. METTL14-mediated N6-methyladenosine modification of Col17a1/Itgα6/Itgβ4 governs epidermal homeostasis [MeRIP-seq]

To elucidate the role of METTL14-mediated m6A modification in epidermal development, we conditionally knocked out the m6A methyltransferase METTL14 in mouse epidermal keratinocytes. We then performed MeRIP-seq analysis of epidermal keratinosis from P0 control mice (K14Cre,Mettl14F/+). Overall design: We performed MeRIP-seq analysis of epidermal keratinosis from P0 control mice (K14Cre,Mettl14F/+).

为阐明METTL14（甲基转移酶样蛋白14）介导的m6A（N6-甲基腺嘌呤）修饰在表皮发育中的调控作用，本研究在小鼠表皮角质形成细胞中条件性敲除m6A甲基转移酶METTL14。随后，我们对出生后0日龄（P0）对照小鼠（K14Cre,Mettl14F/+）的表皮角化组织开展MeRIP-seq（甲基化RNA免疫沉淀测序）分析。实验整体设计：我们对出生后0日龄（P0）对照小鼠（K14Cre,Mettl14F/+）的表皮角化组织开展MeRIP-seq（甲基化RNA免疫沉淀测序）分析。