Additional file 5 of The intrinsically disordered region of GCE protein adopts a more fixed structure by interacting with the LBD of the nuclear receptor FTZ-F1

Additional file 5: Figure S5. Subcellular localization of GCEC and FTZ-F1. The subcellular distribution of the YFP-GCEC-FLAG and YFP-LBD FTZ-F1 was analyzed 24 h after the transfection or co-transfection of the COS-7 cells. A) Representative images of the COS-7 cells expressing the YFP-GCEC-FLAG or CFP-LBD FTZ-F1 after single transfection. The YFP-GCEC-FLAG was observed in nuclei, while the CFP-LBD FTZ-F1 localized within the whole cell. B) Representative images of the COS-7 cells expressing the YFP-GCEC-FLAG and CFP-LBD FTZ-F1 after co-transfection. The YFP-GCEC-FLAG was still observed in the nuclei, while the CFP-LBD FTZ-F1 was shifted to a predominantly nuclear localization. C) Representative images of the YFP and CFP expression used as the control. Both proteins were observed within the whole cell.

附加文件5：图S5。GCEC与FTZ-F1的亚细胞定位。本研究对转染或共转染COS-7细胞24小时后的黄色荧光蛋白（Yellow Fluorescent Protein，YFP）标记的GCEC-FLAG融合蛋白（YFP-GCEC-FLAG）以及YFP标记的配体结合域（Ligand Binding Domain，LBD）-FTZ-F1融合蛋白（YFP-LBD FTZ-F1）的亚细胞分布情况进行了分析。A）单次转染后，表达YFP-GCEC-FLAG或青色荧光蛋白（Cyan Fluorescent Protein，CFP）标记的LBD-FTZ-F1融合蛋白（CFP-LBD FTZ-F1）的COS-7细胞代表性成像结果：YFP-GCEC-FLAG定位于细胞核内，而CFP-LBD FTZ-F1则均匀分布于整个细胞。B）共转染YFP-GCEC-FLAG与CFP-LBD FTZ-F1的COS-7细胞代表性成像结果：YFP-GCEC-FLAG仍定位于细胞核内，而CFP-LBD FTZ-F1则主要移位至细胞核区域。C）以YFP与CFP表达作为对照的代表性成像结果：两种蛋白均均匀分布于整个细胞。