Evaluation of nanopore sequencing to increase accessibility to eDNA studies in biodiverse countries

In this study we sampled environmental DNA from 5 water bodies within 2 national parks in eastern Zambia. We amplified a 97bp fragment of the 12s ribosomal gene using vertebrate specific primers. We sequenced the pool of 96 PCR products with both nanopore and Illumina.

本研究从赞比亚东部2座国家公园内的5处水体中采集了环境DNA（environmental DNA）。我们使用脊椎动物特异性引物扩增了12S核糖体基因（12S ribosomal gene）的97bp片段。我们采用纳米孔（nanopore）与Illumina两种测序平台，对96份聚合酶链式反应（PCR）产物混合池进行了测序。